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They received standard nutrition (pelleted feed) and fresh water under free access (ad libitum) in accordance with Good Laboratory Practice (GLP)

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MODE 3 Time-Course Metabolic Perturbation Profiling Samples collected at multiple time points after compound addition reveal the temporal sequence of metabolic pathway involvement distinguishing primary metabolic effects (rapid, often within 14 hours) from secondary and adaptive responses

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This is where retatrutide separates itself from single and dual receptor agonists